ha cd44 interaction inhibitor 2 Search Results


ha cd44  (MedChemExpress)
94
MedChemExpress ha cd44
Endothelial cells promote mitochondrial function and extracellular matrix gene expression of endometrial cells within the dynamically cultured HEO complex.a. Comparison of expression levels of MT-RNR1, MT-RNR2, and MT-ND4L genes between the dynamically cultured EO and HEO complexes in different clusters.b. EEOs were isolated from the dynamically cultured EO and HEO complexes using a mouth pipette. Comparison of MTRNR1, MTRNR2, and MT-ND4L genes in EO and HEO complexes. GAPDH was used as the internal control. ∗∗P < 0.01, ∗∗∗P < 0.001, Student's t-test.c. Flow cytometry analysis showing JC-1 aggregates of mitochondria in the dynamically cultured EO and HEO complexes.d. Ultrastructure of mitochondrion in the dynamically cultured EO and HEO complexes by transmission electron microscopy. Triangle, nucleus; arrow, mitochondrion. Scale bar: 5 μm.e. Flow cytometry analysis of mitochondrial JC-1 aggregates in the dynamically cultured EO and HEO complexes. In the HEO complex, inhibitors of BMP6 (dorsomorphin, 10 μM) and <t>CD44</t> <t>(HA-CD44,</t> 10 μM) were used to inhibit the respective receptors of EEOs. In the EO complex, recombinant proteins BMP6 (50 ng/mL) or Galectin-9 (10 ng/mL) were introduced as ligands.f. Quantitative results of flow cytometry assays in panel e. ∗P < 0.05, ∗∗∗P < 0.001, one-way ANOVA.g. The electrical resistance, measured using the EVOM2 voltohmmeter in Transwell inserts, reflects the growth of cells in HEO and EO complexes treated with inhibitors and recombinant proteins, respectively. ∗∗∗P < 0.001, two-way ANOVA.h. Comparison of the gene expression level of MMPs (MMP1 and MMP3) and collagens (COL1A1, COL4A1 and COL5A3) between the dynamically cultured EO and HEO complexes in different clusters.i. Gene expression levels of MMP1 and MMP3 in EEOs isolated from the dynamically cultured HEO complex relative to that from the dynamically cultured EO complex. 18S was used as internal control. ∗∗∗P < 0.001, Student's t-test.j. MMP and collagen expression in EO and HEO complexes under estrogen treatment. Left: Representative western blots of MMP1, MMP3, COL1A1, and COL4A1. β-ACTIN was used as loading control. Right: Quantitative analysis of protein expression levels (normalized to β-ACTIN), measured using ImageJ software.Data presented as mean ± SD; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 (Student's t -test or one-way ANOVA).
Ha Cd44, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ha-cd44 interaction inhibitor 2  (MuseChem Chemicals)
N/A
HA-CD44 Interaction Inhibitor 2(CAT: I041004), also known as Antitumor agent-108 (compound 5), is a small molecule designed to disrupt the interaction between hyaluronic acid (HA) and the CD44 receptor. This interaction plays a crucial role
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ha-cd44 interaction inhibitor 2  (TargetMol)
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Endothelial cells promote mitochondrial function and extracellular matrix gene expression of endometrial cells within the dynamically cultured HEO complex.a. Comparison of expression levels of MT-RNR1, MT-RNR2, and MT-ND4L genes between the dynamically cultured EO and HEO complexes in different clusters.b. EEOs were isolated from the dynamically cultured EO and HEO complexes using a mouth pipette. Comparison of MTRNR1, MTRNR2, and MT-ND4L genes in EO and HEO complexes. GAPDH was used as the internal control. ∗∗P < 0.01, ∗∗∗P < 0.001, Student's t-test.c. Flow cytometry analysis showing JC-1 aggregates of mitochondria in the dynamically cultured EO and HEO complexes.d. Ultrastructure of mitochondrion in the dynamically cultured EO and HEO complexes by transmission electron microscopy. Triangle, nucleus; arrow, mitochondrion. Scale bar: 5 μm.e. Flow cytometry analysis of mitochondrial JC-1 aggregates in the dynamically cultured EO and HEO complexes. In the HEO complex, inhibitors of BMP6 (dorsomorphin, 10 μM) and CD44 (HA-CD44, 10 μM) were used to inhibit the respective receptors of EEOs. In the EO complex, recombinant proteins BMP6 (50 ng/mL) or Galectin-9 (10 ng/mL) were introduced as ligands.f. Quantitative results of flow cytometry assays in panel e. ∗P < 0.05, ∗∗∗P < 0.001, one-way ANOVA.g. The electrical resistance, measured using the EVOM2 voltohmmeter in Transwell inserts, reflects the growth of cells in HEO and EO complexes treated with inhibitors and recombinant proteins, respectively. ∗∗∗P < 0.001, two-way ANOVA.h. Comparison of the gene expression level of MMPs (MMP1 and MMP3) and collagens (COL1A1, COL4A1 and COL5A3) between the dynamically cultured EO and HEO complexes in different clusters.i. Gene expression levels of MMP1 and MMP3 in EEOs isolated from the dynamically cultured HEO complex relative to that from the dynamically cultured EO complex. 18S was used as internal control. ∗∗∗P < 0.001, Student's t-test.j. MMP and collagen expression in EO and HEO complexes under estrogen treatment. Left: Representative western blots of MMP1, MMP3, COL1A1, and COL4A1. β-ACTIN was used as loading control. Right: Quantitative analysis of protein expression levels (normalized to β-ACTIN), measured using ImageJ software.Data presented as mean ± SD; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 (Student's t -test or one-way ANOVA).

Journal: Bioactive Materials

Article Title: Microfluidic chip-integrated vascularized endometrial complexes: Mitochondrial function and paracrine crosstalk enhance regenerative potential

doi: 10.1016/j.bioactmat.2025.08.035

Figure Lengend Snippet: Endothelial cells promote mitochondrial function and extracellular matrix gene expression of endometrial cells within the dynamically cultured HEO complex.a. Comparison of expression levels of MT-RNR1, MT-RNR2, and MT-ND4L genes between the dynamically cultured EO and HEO complexes in different clusters.b. EEOs were isolated from the dynamically cultured EO and HEO complexes using a mouth pipette. Comparison of MTRNR1, MTRNR2, and MT-ND4L genes in EO and HEO complexes. GAPDH was used as the internal control. ∗∗P < 0.01, ∗∗∗P < 0.001, Student's t-test.c. Flow cytometry analysis showing JC-1 aggregates of mitochondria in the dynamically cultured EO and HEO complexes.d. Ultrastructure of mitochondrion in the dynamically cultured EO and HEO complexes by transmission electron microscopy. Triangle, nucleus; arrow, mitochondrion. Scale bar: 5 μm.e. Flow cytometry analysis of mitochondrial JC-1 aggregates in the dynamically cultured EO and HEO complexes. In the HEO complex, inhibitors of BMP6 (dorsomorphin, 10 μM) and CD44 (HA-CD44, 10 μM) were used to inhibit the respective receptors of EEOs. In the EO complex, recombinant proteins BMP6 (50 ng/mL) or Galectin-9 (10 ng/mL) were introduced as ligands.f. Quantitative results of flow cytometry assays in panel e. ∗P < 0.05, ∗∗∗P < 0.001, one-way ANOVA.g. The electrical resistance, measured using the EVOM2 voltohmmeter in Transwell inserts, reflects the growth of cells in HEO and EO complexes treated with inhibitors and recombinant proteins, respectively. ∗∗∗P < 0.001, two-way ANOVA.h. Comparison of the gene expression level of MMPs (MMP1 and MMP3) and collagens (COL1A1, COL4A1 and COL5A3) between the dynamically cultured EO and HEO complexes in different clusters.i. Gene expression levels of MMP1 and MMP3 in EEOs isolated from the dynamically cultured HEO complex relative to that from the dynamically cultured EO complex. 18S was used as internal control. ∗∗∗P < 0.001, Student's t-test.j. MMP and collagen expression in EO and HEO complexes under estrogen treatment. Left: Representative western blots of MMP1, MMP3, COL1A1, and COL4A1. β-ACTIN was used as loading control. Right: Quantitative analysis of protein expression levels (normalized to β-ACTIN), measured using ImageJ software.Data presented as mean ± SD; ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 (Student's t -test or one-way ANOVA).

Article Snippet: Briefly, the dynamically cultured HEO and EO complexes were cultured in eCHIP for 3 days with treatments including dorsomorphin (10 μM, HY-13418, MCE), HA-CD44 (10 μM, HY-149897, MCE), BMP-6 (50 ng/mL, HY-P700029AF, MCE), Galectin-9 (10 ng/mL, C808, Novoprotein, Jiangsu, China), or DMSO (PWL064, Meilunbio) as a negative control.

Techniques: Gene Expression, Cell Culture, Comparison, Expressing, Isolation, Transferring, Control, Flow Cytometry, Transmission Assay, Electron Microscopy, Recombinant, Western Blot, Software